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6 results on '"Foray, Nicolas"'

2. X-rays-Induced Bystander Effect Consists in the Formation of DNA Breaks in a Calcium-Dependent Manner: Influence of the Experimental Procedure and the Individual Factor

Author

Restier-Verlet, Juliette, primary, Joubert, Aurélie, additional, Ferlazzo, Mélanie L., additional, Granzotto, Adeline, additional, Sonzogni, Laurène, additional, Al-Choboq, Joëlle, additional, El Nachef, Laura, additional, Le Reun, Eymeric, additional, Bourguignon, Michel, additional, and Foray, Nicolas, additional

Published
2023
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3. Molecular Influence of the ATM Protein in the Treatment of Human Cells with Different Radioprotective Drugs: Comparisons between Antioxidative and Pro-Episkevic Strategies

Author

Restier-Verlet, Juliette, primary, Drouet, Michel, additional, Pras, Pauline, additional, Ferlazzo, Mélanie L., additional, Granzotto, Adeline, additional, Sonzogni, Laurène, additional, Al-Choboq, Joëlle, additional, El Nachef, Laura, additional, François, Sabine, additional, Bourguignon, Michel, additional, and Foray, Nicolas, additional

Published
2023
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5. DNA Double-Strand Breaks Induced in Human Cells by Twelve Metallic Species: Quantitative Inter-Comparisons and Influence of the ATM Protein

Author

Viau, Muriel, primary, Sonzogni, Laurène, additional, Ferlazzo, Mélanie L., additional, Berthel, Elise, additional, Pereira, Sandrine, additional, Bodgi, Larry, additional, Granzotto, Adeline, additional, Devic, Clément, additional, Fervers, Béatrice, additional, Charlet, Laurent, additional, and Foray, Nicolas, additional

Published
2021
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6. When Chromatin Decondensation Affects Nuclear γH2AX Foci Pattern and Kinetics and Biases the Assessment of DNA Double-Strand Breaks by Immunofluorescence.

Author

Granzotto A, El Nachef L, Restier-Verlet J, Sonzogni L, Al-Choboq J, Bourguignon M, and Foray N

Subjects
Humans, Kinetics, Cell Nucleus metabolism, Fibroblasts metabolism, DNA Repair, Histones metabolism, DNA Breaks, Double-Stranded radiation effects, Chromatin metabolism, Fluorescent Antibody Technique
Abstract

Immunofluorescence with antibodies against phosphorylated forms of H2AX (γH2AX) is revolutionizing our understanding of repair and signaling of DNA double-strand breaks (DSBs). Unfortunately, the pattern of γH2AX foci depends upon a number of parameters (nature of stress, number of foci, radiation dose, repair time, cell cycle phase, gene mutations, etc…) whose one of the common points is chromatin condensation/decondensation. Here, we endeavored to demonstrate how chromatin conformation affects γH2AX foci pattern and influences immunofluorescence signal. DSBs induced in non-transformed human fibroblasts were analyzed by γH2AX immunofluorescence with sodium butyrate treatment of chromatin applied after the irradiation that decondenses chromatin but does not induce DNA breaks. Our data showed that the pattern of γH2AX foci may drastically change with the experimental protocols in terms of size and brightness. Notably, some γH2AX minifoci resulting from the dispersion of the main signal due to chromatin decondensation may bias the quantification of the number of DSBs. We proposed a model called "Christmas light models" to tentatively explain this diversity of γH2AX foci pattern that may also be considered for any DNA damage marker that relocalizes as nuclear foci.

Published
2024
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