1. Synthesis of oligonucleotides containing novel G-clamp analogue with C8-tethered group in phenoxazine ring: Implication to qPCR detection of the low-copy Kemerovo virus dsRNA.
- Author
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Varizhuk, Anna M., Zatsepin, Timofei S., Golovin, Andrey V., Belyaev, Evgeny S., Kostyukevich, Yury I., Dedkov, Vladimir G., Shipulin, German A., Shpakovski, George V., and Aralov, Andrey V.
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DOUBLE-stranded RNA , *OLIGONUCLEOTIDE synthesis , *POLYMERASE chain reaction , *COMPLEMENTARY DNA , *DNA primers - Abstract
Nowadays modified oligonucleotides are widely used in diagnostics and as novel therapeutics. Introduction of modified or unnatural residues into oligonucleotides allows fine tuning of their binding properties to complementary nucleic acids and leads to improved stability both in vitro and in vivo . Previously it was demonstrated that insertion of phenoxazine nucleotides with various groups in C9-position into oligonucleotides leads to a significant increase of duplex stability with complementary DNA and RNA. Here the synthesis of a novel G-clamp nucleoside analogue (G 8AE -clamp) bearing 2-aminoethyl tether at C8-atom is presented. Introduction of such modified residues into oligonucleotides lead to enhanced specificity of duplex formation towards complementary DNA and RNA targets with increased thermal and 3′-exonuclease stability. According to CD-spectroscopy studies G 8AE -clamp does not substantially disrupt helix geometry. Primers containing G 8AE -clamp demonstrated superior sensitivity in qPCR detection of dsRNA of Kemerovo virus in comparison to native oligonucleotides. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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