1. Efficient synthesis of trisaccharide saponins and their tumor cell killing effects through oncotic necrosis
- Author
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Gao, Jian, Li, Xia, Gu, Guofeng, Sun, Bin, Cui, Min, Ji, Mei, and Lou, Hong-Xiang
- Subjects
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CELL-mediated cytotoxicity , *SACCHARIDES , *ORGANIC synthesis , *SAPONINS , *CELL membranes , *CELL death , *CYTOSKELETON - Abstract
Abstract: To investigate the relationship of cytotoxicity and saponins with varied aglycones, based on the structure of indioside E 1, a natural derived anti-tumor active ingredient from Chinese medicinal plant Solanum indicum L., five novel saponins 2–6 bearing the same trisaccharide moiety together with 1 were efficiently synthesized via a transglycosylation strategy. MTT assay revealed the killing effects to tumor cells of the synthesized saponins are varied with the change of aglycones. Furthermore, time-lapse microscopy, LDH release, PI staining, and immunocytochemical investigations demonstrated that the cell death caused by neosaponin 2 was through oncotic necrosis involving plasma membrane perturbation and destruction of cytoskeleton. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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