1. "Cut and combine": an easy membrane-supported combinatorial synthesis technique.
- Author
-
Dittrich F, Tegge W, and Frank R
- Subjects
- Amino Acid Sequence, Biotinylation, Cyclic AMP-Dependent Protein Kinases metabolism, Indicators and Reagents, Oligopeptides chemistry, Paper, Protein Conformation, Streptavidin, Structure-Activity Relationship, Substrate Specificity, Drug Design, Models, Molecular, Oligopeptides chemical synthesis, Peptide Library
- Abstract
A combinatorial synthesis process involving sequential cycles of cutting a membrane support into pieces and combining these into groups and subjecting the groups to simultaneous solid-phase chemical reactions is demonstrated by the rapid assembly of four hundred N-terminally biotinylated, soluble, octameric peptide pools. Index patterns printed onto the synthesis membrane allowed a direct identification of the compounds. These were used to study protein kinase substrate selection in a parallel microplate adapted 32P-phosphorylation assay with subsequent spotting on a biotin-capture membrane.
- Published
- 1998
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