1. Hemin-incorporated nanoflowers as enzyme mimics for colorimetric detection of foodborne pathogenic bacteria.
- Author
-
Wang KY, Bu SJ, Ju CJ, Li CT, Li ZY, Han Y, Ma CY, Wang CY, Hao Z, Liu WS, and Wan JY
- Subjects
- Animals, Benzothiazoles chemistry, Foodborne Diseases microbiology, Humans, Hydrogen Peroxide chemistry, Immunoassay methods, Limit of Detection, Milk microbiology, Peroxidase chemistry, Sulfonic Acids chemistry, Biomimetic Materials chemistry, Biosensing Techniques methods, Colorimetry methods, Escherichia coli Infections microbiology, Escherichia coli O157 isolation & purification, Hemin chemistry, Nanostructures chemistry
- Abstract
Rapid, sensitive and point-of-care detection of foodborne pathogenic bacteria is essential for food safety. In this study, we found that hemin-concanavalin A hybrid nanoflowers (HCH nanoflowers), as solid mimic peroxidase, could catalyze oxidation of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) in the presence of H
2 O2 to a green-colored product. HCH nanoflowers, integrating the essential functions of both biological recognition and signal amplification, meet the requirements of signal labels for colorimetric immunoassay of bacteria. In view of the excellent peroxidase mimetic catalytic activity of HCH nanoflowers, a colorimetric biosensing platform was newly constructed and applied for sensitive detection of foodborne Escherichia coli O157:H7 (E. coli O157:H7). The corresponding detection limits was as low as 4.1 CFU/mL with wide linear ranges (101 -106 CFU/mL)., (Copyright © 2018. Published by Elsevier Ltd.)- Published
- 2018
- Full Text
- View/download PDF