1. Antibodies under pressure: A Small-Angle X-ray Scattering study of Immunoglobulin G under high hydrostatic pressure
- Author
-
Karin Julius, Matthias Voetz, Michael Paulus, Julian Schulze, Nico König, and Metin Tolan
- Subjects
0301 basic medicine ,Hydrostatic pressure ,Biophysics ,Crystal structure ,Biochemistry ,Immunoglobulin G ,law.invention ,03 medical and health sciences ,X-Ray Diffraction ,law ,Scattering, Small Angle ,Spectroscopy, Fourier Transform Infrared ,Hydrostatic Pressure ,Humans ,Molecule ,030102 biochemistry & molecular biology ,biology ,Small-angle X-ray scattering ,Chemistry ,Scattering ,Organic Chemistry ,Crystallography ,030104 developmental biology ,biology.protein ,Radius of gyration ,Hydrostatic equilibrium - Abstract
In the present work two subclasses of the human antibody Immunoglobulin G (IgG) have been investigated by Small-Angle X-ray Scattering under high hydrostatic pressures up to 5kbar. It is shown that IgG adopts a symmetric T-shape in solution which differs significantly from available crystal structures. Moreover, high-pressure experiments verify the high stability of the IgG molecule. It is not unfolded by hydrostatic pressures of up to 5kbar but a slight increase of the radius of gyration was observed at elevated pressures.
- Published
- 2017
- Full Text
- View/download PDF