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Start Over Author "BULONE D" Journal biophysical journal
7 results on '"BULONE D"'

6. Kinetics of Insulin Aggregation: Disentanglement of Amyloid Fibrillation from Large-Size Cluster Formation

Author

Mauro Manno, Donatella Bulone, Pier Luigi San Biagio, Vincenzo Martorana, Emanuela Fabiola Craparo, MANNO M, CRAPARO EF, MARTORANA V, BULONE D, and SAN BIAGIO PL

Subjects
MECHANISM, Models, Molecular, Amyloid, medicine.medical_treatment, Kinetics, Biophysics, chemistry.chemical_compound, Exponential growth, FIBRILS, medicine, Cluster (physics), Humans, Insulin, Computer Simulation, Benzothiazoles, Particle Size, ATOMIC-FORCE MICROSCOPY, PATHWAYS, Proteins, Fluorescence, LIGHT-SCATTERING, Crystallography, Thiazoles, chemistry, Models, Chemical, Multiprotein Complexes, Thioflavin, Particle size, BETA-PROTEIN, NUCLEATION
Abstract

Kinetics of human insulin aggregation has been studied at pH 1.6 and 60 degrees C, when amyloid fibrils are formed. We developed a novel approach based on the analysis of scattered light intensity distribution, which allows distinguishing between small and large size aggregates. By this method, we observed an exponential growth of fibrillar aggregates implying a heterogeneous aggregation mechanism. Also, the apparent lag time observed, correlated with the major increase of thioflavin T fluorescence, has been assigned to the onset of large size cluster formation.

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7. Kinetics of insulin aggregation: disentanglement of amyloid fibrillation from large-size cluster formation.

Author

Manno M, Craparo EF, Martorana V, Bulone D, and San Biagio PL

Subjects
Amyloid ultrastructure, Benzothiazoles, Computer Simulation, Humans, Kinetics, Multiprotein Complexes chemistry, Multiprotein Complexes ultrastructure, Particle Size, Amyloid chemistry, Insulin chemistry, Models, Chemical, Models, Molecular, Thiazoles chemistry
Abstract

Kinetics of human insulin aggregation has been studied at pH 1.6 and 60 degrees C, when amyloid fibrils are formed. We developed a novel approach based on the analysis of scattered light intensity distribution, which allows distinguishing between small and large size aggregates. By this method, we observed an exponential growth of fibrillar aggregates implying a heterogeneous aggregation mechanism. Also, the apparent lag time observed, correlated with the major increase of thioflavin T fluorescence, has been assigned to the onset of large size cluster formation.

Published
2006
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