1. Flow Injection of DNA in Nanopores : Direct Optical Visualization of a Pressure Threshold
- Author
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Thomas Auger, Virgile Viasnoff, Jérôme Mathé, Loïc Auvray, Fabien Montel, Jean-Marc Di Meglio, Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE - UMR 8587), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université d'Évry-Val-d'Essonne (UEVE)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine, Department of Biological Sciences [Singapore], National University of Singapore (NUS), Mechanobiology Institute [Singapore] (MBI), Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique de l'ENS Lyon (Phys-ENS), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC), and Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
chemistry.chemical_classification ,Quantitative Biology::Biomolecules ,0303 health sciences ,Materials science ,Zero mode ,Biomolecule ,Near-field optics ,Biophysics ,Polymer ,Visualization ,03 medical and health sciences ,Nanopore ,0302 clinical medicine ,Flow (mathematics) ,chemistry ,Chemical physics ,Radius of gyration ,[PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] ,ComputingMilieux_MISCELLANEOUS ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
International audience; In the regime of pores smaller than the radius of gyration, the flow injection of polymers and biopolymers exhibit a flow threshold independent of the pore and of the polymer itself. We have developed a new combination of near field optics (zero mode wave guide) and image analysis in order to revisit this phenomenon. Working at constant pressure we are able to control and observe directly the transport of individual biomolecules with a time resolution of 5 ms. In the case of DNA, we show that the forced transport through the pore can be described as an energetic barrier only dependent on the injected flow. Further application to biological systems of this barrier measurement will also be discussed.
- Published
- 2014
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