1. Single Cell Wall Nonlinear Mechanics Revealed by a Multiscale Analysis of AFM Force-Indentation Curves
- Author
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Françoise Argoul, Mohammed Bendahmane, Alain Arneodo, Annik Berne-Dedieu, Cristina Martinez-Torres, Judit Szécsi, Simona Digiuni, Laboratoire de Physique de l'ENS Lyon (Phys-ENS), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Agence Nationale de la Recherche ANR-10-BLAN-1516 ANR 10-BLANC-1615 ANR-11 IDEX-0007-02, PRES-University of Lyon, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon)
- Subjects
0106 biological sciences ,Cantilever ,Materials science ,[SDV]Life Sciences [q-bio] ,Turgor pressure ,Arabidopsis ,Biophysics ,Nanotechnology ,Microscopy, Atomic Force ,01 natural sciences ,Viscoelasticity ,law.invention ,Biomechanical Phenomena ,03 medical and health sciences ,law ,Indentation ,Molecular Machines, Motors, and Nanoscale Biophysics ,strength of materials ,Composite material ,Elasticity (economics) ,030304 developmental biology ,0303 health sciences ,Viscosity ,cellule végétale ,viscoélasticité ,Elasticity ,résistance mécanique ,Nonlinear system ,plant cells ,cell wall ,Hydrostatic equilibrium ,paroi cellulaire ,010606 plant biology & botany - Abstract
Individual plant cells are rather complex mechanical objects. Despite the fact that their wall mechanical strength may be weakened by comparison with their original tissue template, they nevertheless retain some generic properties of the mother tissue, namely the viscoelasticity and the shape of their walls, which are driven by their internal hydrostatic turgor pressure. This viscoelastic behavior, which affects the power-law response of these cells when indented by an atomic force cantilever with a pyramidal tip, is also very sensitive to the culture media. To our knowledge, we develop here an original analyzing method, based on a multiscale decomposition of force-indentation curves, that reveals and quantifies for the first time the nonlinearity of the mechanical response of living single plant cells upon mechanical deformation. Further comparing the nonlinear strain responses of these isolated cells in three different media, we reveal an alteration of their linear bending elastic regime in both hyper- and hypotonic conditions.
- Published
- 2015
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