1. Estimation of the diversity between DNA calorimetric profiles, differential melting curves and corresponding melting temperatures
- Author
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Chun-Ling Chang, Dmitri Y. Lando, Oleg N. Murashko, Elena N. Galyuk, Inessa E. Grigoryan, Alexander S. Fridman, and Chin-Kun Hu
- Subjects
0301 basic medicine ,Base pair ,Melting temperature ,Organic Chemistry ,Relative standard deviation ,Biophysics ,Analytical chemistry ,Thermodynamics ,Slight change ,Calorimetry, Indirect ,General Medicine ,DNA ,010402 general chemistry ,Nucleic Acid Denaturation ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Biomaterials ,03 medical and health sciences ,Formalism (philosophy of mathematics) ,chemistry.chemical_compound ,030104 developmental biology ,Differential scanning calorimetry ,chemistry - Abstract
The Poland-Fixman-Freire formalism was adapted for modeling of calorimetric DNA melting profiles, and applied to plasmid pBR 322 and long random sequences. We studied the influence of the difference (HGC -HAT ) between the helix-coil transition enthalpies of AT and GC base pairs on the calorimetric melting profile and on normalized calorimetric melting profile. A strong alteration of DNA calorimetrical profile with HGC -HAT was demonstrated. In contrast, there is a relatively slight change in the normalized profiles and in corresponding ordinary (optical) normalized differential melting curves (DMCs). For fixed HGC -HAT , the average relative deviation (S) between DMC and normalized calorimetric profile, and the difference between their melting temperatures (Tcal -Tm ) are weakly dependent on peculiarities of the multipeak fine structure of DMCs. At the same time, both the deviation S and difference (Tcal -Tm ) enlarge with the temperature melting range of the helix-coil transition. It is shown that the local deviation between DMC and normalized calorimetric profile increases in regions of narrow peaks distant from the melting temperature.
- Published
- 2016