1. Enhanced fatty acid storage combined with the multi-factor optimization of fermentation for high-level production of docosahexaenoic acid in Schizochytrium sp.
- Author
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Jia, Yu-Lei, Zhang, Ying, Xu, Lu-Wei, Zhang, Zi-Xu, Xu, Ying-Shuang, Ma, Wang, Gu, Yang, and Sun, Xiao-Man
- Subjects
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DOCOSAHEXAENOIC acid , *FATTY acids , *MALIC acid , *CITRIC acid , *RESPONSE surfaces (Statistics) , *FERMENTATION - Abstract
[Display omitted] • High-titer production of DHA was achieved. • Overexpression of diacylglycerol acyltransferase enhanced lipid and DHA titers. • The optimal addition time was 48 h after inoculation. • The maximum DHA titer was 42.23 g/L. Schizochytrium sp. has received much attention for its ability to synthesize and accumulate high-level docosahexaenoic acid (DHA), which can reach nearly 40 % of total fatty acids. In this study, the titer of DHA in Schizochytrium sp. was successfully improved by enhancing DHA storage through overexpressing the diacylglycerol acyltransferase (ScDGAT2C) gene, as well as optimizing the supply of precursors and cofactors required for DHA synthesis by response surface methodology. Notably, malic acid, citric acid, and biotin showed synergistic and time-dependent effects on DHA accumulation. The maximum lipid and DHA titers of the engineered Schizochytrium sp. strain reached 84.28 ± 1.02 g/L and 42.23 ± 0.69 g/L, respectively, with the optimal concentration combination (1.62 g/L malic acid + 0.37 g/L citric acid + 8.28 mg/L biotin) were added 48 h after inoculation. This study provides an effective strategy for improving lipid and DHA production in Schizochytrium sp. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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