1. The identification of and relief from Fe3+ inhibition for both cellulose and cellulase in cellulose saccharification catalyzed by cellulases from Penicillium decumbens.
- Author
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Wang M, Mu Z, Wang J, Hou S, Han L, Dong Y, Xiao L, Xia R, and Fang X
- Subjects
- Cellulase metabolism, Dithiothreitol pharmacology, Edetic Acid pharmacology, Hydrogen Peroxide pharmacology, Hydrolysis drug effects, Ions, Penicillium drug effects, Biocatalysis drug effects, Carbohydrate Metabolism drug effects, Cellulase antagonists & inhibitors, Cellulose metabolism, Iron pharmacology, Penicillium enzymology
- Abstract
Lignocellulosic biomass is an underutilized, renewable resource that can be converted to biofuels. The key step in this conversion is cellulose saccharification catalyzed by cellulase. In this work, the effect of metal ions on cellulose hydrolysis by cellulases from Penicillium decumbens was reported for the first time. Fe(3+) and Cu(2+) were shown to be inhibitory. Further studies on Fe(3+) inhibition showed the inhibition takes place on both enzyme and substrate levels. Fe(3+) treatment damages cellulases' capability to degrade cellulose and inhibits all major cellulase activities. Fe(3+) treatment also reduces the digestibility of cellulose, due to its oxidation. Treatment of Fe(3+)-treated cellulose with DTT and supplementation of EDTA to saccharification systems partially relieved Fe(3+) inhibition. It was concluded that Fe(3+) inhibition in cellulose degradation is a complicated process in which multiple inhibition events occur, and that relief from Fe(3+) inhibition can be achieved by the supplementation of reducing or chelating agents., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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