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Hydrogenotrophic denitrification (HD) is a promising autotrophic biological process for advanced nitrogen removal, while sludge granulation was seldom reported. This study aimed to cultivate granular sludge to improve capacity and stability of HD process. The resulting HD granular sludge performed high nitrogen removal rate (NRR) of 0.42 ± 0.0.4 kgN/(m

Heterotrophic bacteria (HB) are generally prevalent in anammox-based processes, but their functional and ecological roles in partial nitritation-anammox (PN/A) process treating high-organics wastewater remained unclear. This study aimed to elucidate HB activities and microbial interactions in a one-stage PN/A treating thermal hydrolysis process (THP) - anaerobic digestion (AD) reject water. The PN/A reactor achieved a satisfactory nitrogen removal rate of 0.58 ± 0.06 g N/(L·d), and around 12% of COD in the THP-AD reject water was removed. N2O emission factors of the PN/A reactor were 1.15% ± 0.18% treating synthetic wastewater, and 0.95% ± 0.06% treating reject water. A balanced symbiotic relationship was maintained between HB and functional groups (i.e., anammox bacteria and aerobic-ammonia-oxidizing bacteria) over the reactor operation. The relative abundances of Anaerolineae spp. clearly increased, while Denitratisoma, capable of denitrification, slightly decreased when treating THP-AD reject water. The preference for electron donors of heterotrophs explained discrepant growth trends.

Lignin removal is essential for value-added utilization of hemicelluloses and acetic acid present in the prehydrolysis liquor (PHL) of a kraft-based hardwood dissolving pulp production. In this paper, a novel process concept, consisting of laccase-induced lignin polymerization, followed by filtration/flocculation, was developed to enhance the lignin removal. The results showed that the lignin removal increased from 11% to 46–61% at laccase concentration of 1–4 U mL−1. The GPC results showed that the molecular weight of the lignin from the laccase treated PHL was increased by 160% in comparison with the original one. The subsequent flocculation using singular Poly-DADMAC system or dual polymer system of Poly-DADMAC/CPAM can further remove 10–15% lignin. The concentrations of hemicelluloses and acetic acid were negligibly affected during the laccase treatment, while flocculation caused 12–15% of total sugar loss. Additionally, the process incorporates this new concept into the kraft-based dissolving pulp production process was proposed.

The commercial pre-hydrolysis kraft-based dissolving pulp production process can be a typical example for the demonstration/implementation of the integrated forest biorefinery concept. In this study, the concept of cellulase treatment of this dissolving pulp for enhancement of accessibility/reactivity in terms of viscose rayon production was demonstrated. The cellulase treatment resulted in the formation of additional openings/surface areas in the fiber structure via the possible action of "etching". As a result, the pore volume of pulp fibers increased, which led to the increase in the accessibility to xanthation, and thus Fock reactivity. Results showed that the cellulase treatment was effective in increasing the Fock reactivity, at a cellulase dosage of 2u/g (based on the dry weight of pulp), the Fock reactivity increased from 47.67% to 79.9%. The adoption of cellulase treatment to hardwood kraft-based dissolving pulp can provide an efficient approach for enhancing its performance in the commercial viscose-rayon process.