1. Polyhydroxybutyrate production by direct use of waste activated sludge in phosphorus-limited fed-batch culture
- Author
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Etienne Paul, Jean-Louis Uribelarrea, Guillermina Hernandez-Raquet, Anne-Sophie Lepeuple, Laëtitia Cavaillé, Estelle Grousseau, Mathieu Pocquet, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche sur l’Eau, Veolia Environnement, French National Research Agency (VALORIA project), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Environmental Engineering ,Time Factors ,[SDV]Life Sciences [q-bio] ,Polyesters ,chemistry.chemical_element ,Bioengineering ,macromolecular substances ,010501 environmental sciences ,01 natural sciences ,Acclimatization ,Polyhydroxybutyrate ,Direct accumulation ,Acetic acid ,chemistry.chemical_compound ,010608 biotechnology ,RNA, Ribosomal, 16S ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,Food science ,Waste activated sludge ,Polyhydroxybutyrate (PHB) ,Waste Management and Disposal ,0105 earth and related environmental sciences ,2. Zero hunger ,Biological Oxygen Demand Analysis ,biology ,Bacteria ,Sewage ,Renewable Energy, Sustainability and the Environment ,business.industry ,Phosphorus ,technology, industry, and agriculture ,General Medicine ,biology.organism_classification ,Biotechnology ,Fed-batch culture ,Butyrates ,Activated sludge ,chemistry ,Microbial population biology ,P limitation ,Batch Cell Culture Techniques ,Feasibility Studies ,lipids (amino acids, peptides, and proteins) ,business - Abstract
International audience; Polyhydroxybutyrate (PHB) production directly by waste activated sludge (WAS) was investigated in aerobic fed-batch conditions using acetic acid as substrate. PHB production was induced by phosphorus limitation. WAS of different origin were tested with various degrees of phosphorus limitation and PHB contents of up to 70% (gCOD(PHB)/gCOD(particulate)) were obtained. This strategy showed the importance of maintaining cell growth for PHB production in order to increase PHB concentration and that the degree of phosphorus limitation has a direct impact on the quantity of PHB produced. Pyrosequencing of 165 rRNA transcripts showed changes in the active bacteria of the WAS microbial community as well as the acclimation of populations depending on sludge origin. The monitoring of the process appeared as the key factor for optimal PHB production by WAS. Different strategies are discussed and compared in terms of carbon yield and PHB content with the feast and famine selection process.
- Published
- 2013
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