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The study investigated the inactivation of Microcystis aeruginosa using a combined approach involving thermally activated peroxyacetic acid (Heat/PAA) and thermally activated persulfate (Heat/PDS). The Heat/PDS algal inactivation process conforms to first-order reaction kinetics. Both hydroxyl radical (•OH) and sulfate radical (SO 4 - •) significantly impact the disruption of cell integrity, with SO 4 - • assuming a predominant role. PAA appears to activate organic radicals (RO•), hydroxyl (•OH), and a minimal amount of singlet oxygen ( 1 O 2 ). A thorough analysis underscores persulfate's superior ability to disrupt algal cell membranes. Additionally, SO 4 - • can convert small-molecule proteins into aromatic hydrocarbons, accelerating cell lysis. PAA can accelerate cell death by diffusing into the cell membrane and triggering advanced oxidative reactions within the cell. This study validates the effectiveness of the thermally activated persulfate process and the thermally activated peroxyacetic acid as strategies for algae inactivation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This study utilized corn straw as the feedstock to synthesize biochar (BC) loaded with cobalt-zeolitic imidazolate framework nanoparticles and boron nitride quantum dots. The prepared BC composite, named BN 3 Z 0.5 BC, efficiently activated peracetic acid (PAA), resulting in the degradation of 94.8% of sulfadiazine (SDZ) in five minutes. Compared to pure BC, the SDZ removal rate increased nearly 5-fold. Mechanism analysis revealed that the main degradation pathway involves synergism between free and non-free radicals. The defect structure on the BC surface possesses a high charge density, stimulating PAA to produce more active species, while nitrogen-oxygen vacancy formation significantly promotes charge transfer. Besides, the unique structure of BC ensures good stability and recyclability, effectively controlling metal leaching. The BN 3 Z 0.5 BC/PAA system shows promising applicability across various water matrices, indicating a favorable application outlook., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This study explores the simultaneous sulfamethoxazole (SMX) removal and short-chain fatty acids (SCFAs) production by a Clostridium sensu stricto-dominated microbial consortium. SMX is a commonly prescribed and persistent antimicrobial agent frequently detected in aquatic environments, while the prevalence of antibiotic-resistant genes limits the biological removal of SMX. Under strictly anaerobic conditions, sequencing batch cultivation coupled with co-metabolism resulted in the production of butyric acid, valeric acid, succinic acid, and caproic acid. Continuous cultivation in a CSTR achieved a maximum butyric acid production rate and yield of 0.167 g/L/h and 9.56 mg/g COD, respectively, while achieving a maximum SMX degradation rate and removal capacity of 116.06 mg/L/h and 55.8 g SMX/g biomass. Furthermore, continuous anaerobic fermentation reduced sul genes prevalence, thus limiting the transmission of antibiotic resistance genes during antibiotic degradation. These findings suggest a promising approach for efficient antibiotic elimination while simultaneously producing valuable products (e.g., SCFAs)., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

A sequential anaerobic digestion and phycoremediation process was employed to recover nutrients and remove pollutants from dairy wastewater (DW), while simultaneously producing biomethane and biochemicals. Anaerobic digestion of 100% DW achieved a methane content and production rate of 53.7% and 0.17 L/L/d, respectively. This was accompanied by the removal of 65.5% chemical oxygen demand (COD), 86% total solid (TS), and 92.8% volatile fatty acids (VFAs). The anaerobic digestate was then used to grow Chlorella sorokiniana SU-1. Using 25% diluted digestate as the medium, SU-1 could reach 4.64 g/L biomass concentration, with total nitrogen (TN), total phosphorus (TP) and COD removal efficiencies of 77.6%, 87.1% and 70.4%, respectively. The obtained microalgal biomass (contained 38.5% carbohydrates, 24.9% proteins, 8.8% lipids) was used to co-digest with DW, resulting in good methane production performance. Co-digestion with 25% (w/v) algal biomass obtained a higher CH 4 content (65.2%) and production rate (0.16 L/L/d) than other ratios., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

To preserve the water resources, this study has analyzed the ecotoxicity and antibiotic resistance genes (ARGs) induction capacity of sulfadiazine degradation intermediates resulting from persulfate activation oxidation enhanced by ultraviolet, ultrasound and microwave. The five degradation pathways caused by the contribution discrepancy of electron transfer and singlet oxygen ( 1 O 2 ) and variations in the ecotoxicity of different degradation products were analyzed. Microcosm experiment exhibited that the microbial community in actual water changed significantly with SDZ and degradation intermediates, in which the dominant genera were Aeromonas, Cupriavidus, Elizabethkingia and Achromobacter. Except for the selective pressure on bacteria, the degradation intermediates also exert a certain degree or even stronger induction on sulfonamide ARGs (sul4, sul1 and sul2) than SDZ. Furthermore, the potential hosts for sulfonamide ARGs were revealed by network analysis. These results provide a better understanding of antibiotics degradation mechanism and ARGs occurrence, which is useful for controlling the spread of ARGs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Chlorella sorokiniana has received particular attention as a promising candidate for microalgal biomass and lutein production. In this work, heterotrophic cultivation was explored to improve the lutein production efficiency of a lutein-rich microalga C. sorokiniana FZU60. Flask cultivation results showed that the highest lutein productivity was achieved at 30°C with an initial cell concentration of 1.40 g/L. Furthermore, six types of fed-batch strategies based on nutrient composition and concentration were examined using a 5 L fermenter. Among them, ultra-high lutein production (415.93 mg/L) and productivity (82.50 mg/L/d) with lutein content of 2.57 mg/g were achieved with fed-batch 3F (i.e., pulse-feeding with concentrated urea-N medium to achieve a 3-fold nutrient concentration). The lutein production performance achieved is much higher than the reported values. This work demonstrates that heterotrophic cultivation of C. sorokiniana FZU60 with the proposed fed-batch strategy could significantly enhance the production performance and the commercial viability of microalgae-derived lutein., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Both co-cultivation and co-substrate addition strategies have exhibited massive potential in microalgae-based antibiotic bioremediation. In this study, glucose and sodium acetate were employed as co-substrate in the cultivation of microalgae-bacteria consortium for enhanced sulfadiazine (SDZ) and sulfamethoxazole (SMX) removal. Glucose demonstrated a two-fold increase in biomass production with a maximum specific growth rate of 0.63 ± 0.01 d -1 compared with sodium acetate. The supplementation of co-substrate enhanced the degradation of SDZ significantly up to 703 ± 18% for sodium acetate and 290 ± 22% for glucose, but had almost no effect on SMX. The activities of antioxidant enzymes, including peroxidase, superoxide dismutase and catalase decreased with co-substrate supplementation. Chlorophyll a was associated with protection against sulfonamides and chlorophyll b might contribute to SDZ degradation. The addition of co-substrates influenced bacterial community structure greatly. Glucose enhanced the relative abundance of Proteobacteria, while sodium acetate improved the relative abundance of Bacteroidetes significantly., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The main purpose of this study was to explore the pretreatment process of corn starch wastewater (CSW) and engineered microalgae cultivation strategy to improve the nutrient recovery from wastewater and the yield of microalgae lutein. One-stage enzymatic hydrolysis utilizing α-amylase and glucoamylase simultaneously was established to efficiently harvest a maximum concentration of reducing sugar content of 7.26 g/L from CSW in 50 min. Lutein yield of 10.96 mg/L was obtained under 24 h continuous illumination with 2200 Lux light intensity. Furthermore, a cyclic feeding cultivation strategy was developed to improve lutein accumulation and COD removal up to 25.9 mg/L and 50.7%, respectively, after three cultivation cycles. Lutein yield of 14.86 mg/L and COD removal efficiency of 73.2% was achieved with further implementation in actual wastewater. This work provided a new perspective in developing the potential of cultivating microalgae with corn starch wastewater to produce high-value lutein., (Copyright © 2022 Elsevier Ltd. All rights reserved.)