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Start Over Author "Tian, Qing" Journal bioresource technology
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8. Enhanced cellulase production by Trichoderma viride in a rotating fibrous bed bioreactor

Author

Tian-Qing Lan, Xiaoguang Liu, Dong Wei, and Shang-Tian Yang

Subjects
Proteomics, Environmental Engineering, Rotation, Bioengineering, Cellulase, Microbiology, Fungal Proteins, Hydrolysis, Bioreactors, Bioreactor, Food science, Cellulose, Waste Management and Disposal, Trichoderma, Gel electrophoresis, Mycelium, biology, Renewable Energy, Sustainability and the Environment, Chemistry, Trichoderma viride, food and beverages, General Medicine, Cells, Immobilized, biology.organism_classification, Saccharum, carbohydrates (lipids), Kinetics, Biofilms, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Yield (chemistry), Fermentation, biology.protein, Carbohydrate Metabolism, Bagasse, Biotechnology
Abstract

Filamentous fungi are widely used to produce cellulase, but how the fermentation conditions affect their production is not well known. In this study, cellulase production by Trichoderma viride in submerged fermentations with free cells in a stirred-tank reactor (STR) and immobilized cells in a rotating fibrous-bed bioreactor (RFBB) were investigated. Compared to free-cell fermentation, immobilized-cell fermentation gave 35.5% higher FPase activity and 69.7% higher saccharification yield of sugarcane bagasse (SCB). The secretory proteins in the fermentation broths were analyzed with two-dimensional gel electrophoresis (2-DE) and MALDI-TOF–TOF mass spectrometry, which identified 24 protein spots with differential expression levels. Among them, cellobiohydrolase CBH II and endoglucanase EG II were highly expressed and secreted in the immobilized-cell fermentation, while the free-cell fermentation produced more CBH І and EG ІV. These results showed that immobilized-cell fermentation with T. viride in the RFBB was advantageous for cellulase production.

Published
2013

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