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This study presents a novel denitratation/anammox biofilter (DABF) for nitrate removal from secondary effluent, where denitratation (NO3–→NO2–) is coupled with anammox (NO2– +NH4+→N2) instead of denitritation (NO2–→N2). Total nitrogen (TN) was removed by 81.90% in this DABF when the average effluent TN concentration was 7.82 mg/L. Meanwhile, organic carbon source consumption and backwash sludge production in the DABF were reduced by 63% and 70%, respectively, compared to conventional denitrifying biofilter (DNBF). Nitrogen banlance analysis indicates that 93% of the nitrogen gas produced in DABF was via anammox. Batch tests confirmed that the DABF biofilm reduced nitrite using ammonium as the electron donor, and accumulated nitrite during denitratation, thus providing nitrite for the anammox bacteria. Moreover, high-throughput sequencing approach also revealed that the anammox bacteria Candidatus Brocadia dominated the community, which could also be responsible for the stable processes in DABF by interacting with the other denitrifying bacteria.

Denitratation (NO3−→NO2−)/anammox is a promising method for anammox application in mainstream wastewater treatment plants (WWTPs) to reduce oxygen and organic matter consumption. Achieving nitrite production via denitratation and controlling denitritation (NO2−→N2) is the basis of the denitratation/anammox process. To control denitritation, the denitrifying biocommunity and growth rate are critically reviewed for biocommunity optimization. Then, the short-term and long-term effects of pH on denitritation were summarized and the possible mechanism was discussed, along with the effect of C/N ratio and organic matter type on denitritation. Meanwhile, the strategies for producing nitrite via controlling denitritation are discussed, as well as the processes for achieving nitrogen removal via denitratation/anammox in WWTPs. Finally, the practical application of denitratation/anammox in a full-scale mainstream WWTP is documented.