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Start Over Author "Yu-tao Wang" Journal bioresources
2 results on '"Yu-tao Wang"'

1. Combined water extraction and sodium chlorite pretreated spent mushroom compost for protease production by separate hydrolysis and fermentation and simultaneous saccharification and co-fermentation

Author

Bin-Bin Hu, Ming-Jun Zhu, Yu-Tao Wang, and Pei Lu

Subjects
Co-fermentation, Environmental Engineering, Protease, biology, medicine.medical_treatment, Sodium chlorite, Bioengineering, Water extraction, Bacillus subtilis, biology.organism_classification, Hydrolysis, chemistry.chemical_compound, chemistry, cardiovascular system, medicine, Spent mushroom compost, Fermentation, Food science, Waste Management and Disposal
Abstract

A process of simultaneous saccharification and protease production was successfully established from spent mushroom compost (SMC) created through edible fungi cultivation. The combined water extraction and sodium chlorite pretreatment significantly (p < 0.05) improved enzymatic digestibility of SMC, which led to a reducing sugar yield of 0.759 g/g that was 12 times higher than raw SMC. The water extract from SMC was recycled for simultaneous saccharification and protease production from pretreated SMC by Bacillus subtilis DES-59, which promoted the protein concentration and neutral protease activity by 21.9% and 11.6%, respectively. The simultaneous saccharification and co-fermentation (SScF) of pretreated SMC by Bacillus subtilis DES-59 produced 5518 U/mL protease, which was superior to the separate hydrolysis and fermentation (SHF) process. Fermentation residues containing Bacillus subtilis cells could be further converted into fertilizer. The closed-loop utilization of SMC was achieved using established processes, which indicates potential for application in future biorefineries.

Published
2020

2. Combined Water Extraction and Sodium Chlorite Pretreated Spent Mushroom Compost for Protease Production by Separate Hydrolysis and Fermentation and Simultaneous Saccharification and Co-fermentation.

Author

Pei Lu, Bin-Bin Hu, Yu-Tao Wang, and Ming-Jun Zhu

Subjects
COMPOSTING, FERMENTATION, SODIUM compounds, BACILLUS subtilis, MUSHROOMS, INTERLEUKIN-9, POLYPHENOL oxidase
Abstract

A process of simultaneous saccharification and protease production was successfully established from spent mushroom compost (SMC) created through edible fungi cultivation. The combined water extraction and sodium chlorite pretreatment significantly (p < 0.05) improved enzymatic digestibility of SMC, which led to a reducing sugar yield of 0.759 g/g that was 12 times higher than raw SMC. The water extract from SMC was recycled for simultaneous saccharification and protease production from pretreated SMC by Bacillus subtilis DES-59, which promoted the protein concentration and neutral protease activity by 21.9% and 11.6%, respectively. The simultaneous saccharification and co-fermentation (SScF) of pretreated SMC by Bacillus subtilis DES-59 produced 5518 U/mL protease, which was superior to the separate hydrolysis and fermentation (SHF) process. Fermentation residues containing Bacillus subtilis cells could be further converted into fertilizer. The closed-loop utilization of SMC was achieved using established processes, which indicates potential for application in future biorefineries. [ABSTRACT FROM AUTHOR]

Published
2020
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