1. Antibodies with potent and broad neutralizing activity against antigenically diverse and highly transmissible SARS-CoV-2 variants
- Author
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Lingshu Wang, Tongqing Zhou, Yi Zhang, Eun Sung Yang, Chaim A. Schramm, Wei Shi, Amarendra Pegu, Olamide K. Oloninyi, Amy Ransier, Samuel Darko, Sandeep R. Narpala, Christian Hatcher, David R. Martinez, Yaroslav Tsybovsky, Emily Phung, Olubukola M. Abiona, Evan M. Cale, Lauren A. Chang, Kizzmekia S. Corbett, Anthony T. DiPiazza, Ingelise J. Gordon, Kwanyee Leung, Tracy Liu, Rosemarie D. Mason, Alexandra Nazzari, Laura Novik, Adam S. Olia, Nicole A. Doria-Rose, Tyler Stephens, Christopher D. Stringham, Chloe Adrienna Talana, I-Ting Teng, Danielle Wagner, Alicia T. Widge, Baoshan Zhang, Mario Roederer, Julie E. Ledgerwood, Tracy J. Ruckwardt, Martin R. Gaudinski, Ralph S. Baric, Barney S. Graham, Adrian B. McDermott, Daniel C. Douek, Peter D. Kwong, John R Mascola, Nancy J. Sullivan, and John Misasi
- Subjects
Titer ,biology ,Immunity ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Mutant ,biology.protein ,Antibody ,IC50 ,Virology ,Neutralization ,In vitro ,Article - Abstract
The emergence of highly transmissible SARS-CoV-2 variants of concern (VOC) that are resistant to therapeutic antibodies highlights the need for continuing discovery of broadly reactive antibodies. We identify four receptor-binding domain targeting antibodies from three early-outbreak convalescent donors with potent neutralizing activity against 12 variants including the B.1.1.7 and B.1.351 VOCs. Two of them are ultrapotent, with sub-nanomolar neutralization titers (IC50 in vitro generation of escape mutants, suggesting potential means to mitigate resistance development. These results define the basis of therapeutic cocktails against VOCs and suggest that targeted boosting of existing immunity may increase vaccine breadth against VOCs.One Sentence SummaryUltrapotent antibodies from convalescent donors neutralize and mitigate resistance of SARS-CoV-2 variants of concern.
- Published
- 2021