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Your search keyword '"WEI GAO"' showing total 2 results
2 results on '"WEI GAO"'

1. Hydroxyurea affects in vitro porcine oocyte maturation through increased apoptosis and oxidative stress

Author

Fu-Shi Quan, Dongxu Wang, Jia-Bao Zhang, Yong-Xun Jin, Siyi Huang, Mingjun Zhang, Wen-Zhi Ren, Jindong Hao, Wei Gao, and Xianfeng Yu

Subjects
0301 basic medicine, Embryology, Swine, Biophysics, Cell Death & Injury, Apoptosis, medicine.disease_cause, Biochemistry, Andrology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Zoology & Marine Biology, Oogenesis, Gene expression, Maturation, medicine, Hydroxyurea, Animals, Blastocyst, Molecular Biology, Research Articles, chemistry.chemical_classification, Reactive oxygen species, Cumulus Cells, Embryo, Cell Biology, Glutathione, Oocyte, In Vitro Oocyte Maturation Techniques, Oxidative Stress, 030104 developmental biology, medicine.anatomical_structure, chemistry, Porcine oocyte, Oocytes, Female, 030217 neurology & neurosurgery, Oxidative stress, Developmental Biology
Abstract

Hydroxyurea (HU) is an FDA-approved drug used to treat a variety of diseases, especially malignancies, but is harmful to fertility. We used porcine oocytes as an experimental model to study the effect of HU during oocyte maturation. Exposure of cumulus–oocyte complexes (COCs) to 20 µM (P

Published
2021

2. Ascorbic acid improves parthenogenetic embryo development through TET proteins in mice.

Author

Wei Gao, Xianfeng Yu, Jindong Hao, Ling Wang, Minghui Qi, Liang Han, Chao Lin, and Dongxu Wang

Subjects
*VITAMIN C, *EMBRYOLOGY, *BLASTOCYST, *POLYMERASE chain reaction, *GENE expression
Abstract

The TET (Ten-Eleven Translocation) proteins catalyze the oxidation of 5mC (5-methylcytosine) to 5hmC (5-hydroxymethylcytosine) and play crucial roles in embryonic development. Ascorbic acid (Vc, Vitamin C) stimulates the expression of TET proteins, whereas DMOG (dimethyloxallyl glycine) inhibits TET expression. To investigate the role of TET1, TET2, and TET3 in PA (parthenogenetic) embryonic development, Vc and DMOG treatments were administered during early embryonic development. The results showed that Vc treatment increased the blastocyst rate (20.73 +- 0.46 compared with 26.57 +- 0.53%). By contrast, DMOG reduced the blastocyst rate (20.73 +- 0.46 compared with 11.18 +- 0.13%) in PA embryos. qRT-PCR (quantitative real-time PCR) and IF (immunofluorescence) staining results revealed that TET1, TET2, and TET3 expressions were significantly lower in PA embryos compared with normal fertilized (Con) embryos. Our results revealed that Vc stimulated the expression of TET proteins in PA embryos. However, treatment with DMOG significantly inhibited the expression of TET proteins. In addition, 5hmC was increased following treatment with Vc and suppressed by DMOG in PA embryos. Taken together, these results indicate that the expression of TET proteins plays crucial roles mediated by 5hmC in PA embryonic development. [ABSTRACT FROM AUTHOR]

Published
2019
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