1. Relationship between ADAMTS14/rs4747096 gene polymorphism and knee osteoarthritis in Chinese population
- Author
-
Cheng Ouyang, Shuxin Ren, and Sen Ma
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,China ,Genotype ,gene polymorphism ,Biophysics ,Single-nucleotide polymorphism ,Biochemistry ,Gastroenterology ,Polymorphism, Single Nucleotide ,knee osteoarthritis ,03 medical and health sciences ,0302 clinical medicine ,ADAMTS Proteins ,Gene Frequency ,Internal medicine ,medicine ,Humans ,Genetic Predisposition to Disease ,Molecular Biology ,Allele frequency ,Genetic Association Studies ,Research Articles ,Aged ,030203 arthritis & rheumatology ,medicine.diagnostic_test ,business.industry ,Cell Biology ,Odds ratio ,Middle Aged ,Osteoarthritis, Knee ,Hardy–Weinberg principle ,ADAMTS14 ,Genotype frequency ,030104 developmental biology ,Erythrocyte sedimentation rate ,Female ,Gene polymorphism ,business ,Research Article ,Hardy-Weinberg - Abstract
To investigate the association between single nucleotide polymorphisms (SNPs) of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) 14 (ADAMTS14) gene and susceptibility to knee osteoarthritis (KOA) in Chinese Han population. Using a case–control design, we enrolled 346 KOA patients and 480 healthy controls. Peripheral blood samples were extracted from each subject. Genotype was determined by sequencing PCR products. The genotype frequencies between cases and controls were compared. The genotype distribution was in accordance with Hardy–Weinberg equilibrium. The minor G allele in case group was significantly higher than in the control group (21.4 compared with 8.8%, P=0.000, odds ratio (OR) = 1.71 (95% confidence interval (CI): 1.39–2.11). The GG genotype and the GG/AG combination were more common in the osteoarthritis (OA) group than in the control group. Compared with AA genotype, the GG (OR = 3.09, 95%CI: 2.01–4.75), AG (OR = 2.55, 95%CI: 1.64–3.96), and GG/AG (OR = 1.57, 95%CI: 1.19–2.07) increased the risk of OA. Multiple logistic confirmed the findings by adjusting some potential factors. Subgroup analysis indicated that the ras4747096 was still significantly associated with KOA. There were no significant differences in allele frequency or genotypes frequency for erythrocyte sedimentation rate and C-reaction protein in OA patients (P>0.05). ADAMTS14 gene polymorphism was associated with KOA, and the GG genotype increased the risk of KOA in Chinese Han population. The ADAMTS14 may be a diagnostic marker and therapeutic target for KOA treatment. The future study should explore the specific molecular mechanism.
- Published
- 2018