1. A novel antimicrobial peptide, Ranatuerin-2PLx, showing therapeutic potential in inhibiting proliferation of cancer cells
- Author
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Lei Wang, James F. Burrows, Luyao Zhang, Mei Zhou, Xiaoling Chen, Yingqi Zhang, Xinping Xi, Tianbao Chen, and Chengbang Ma
- Subjects
Male ,Methicillin-Resistant Staphylococcus aureus ,0301 basic medicine ,Ranidae ,Antimicrobial peptides ,Biophysics ,Peptide ,Microbial Sensitivity Tests ,Pharmacology ,Biochemistry ,Amphibian Proteins ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,Minimum inhibitory concentration ,Anti-Infective Agents ,Annexin ,Animals ,Humans ,Propidium iodide ,antibacterial properties ,Molecular Biology ,Research Articles ,anti-cancer ,Cell Proliferation ,Skin ,chemistry.chemical_classification ,Prostatic Neoplasms ,Cell Biology ,Antimicrobial ,030104 developmental biology ,chemistry ,Apoptosis ,PC-3 Cells ,Cancer cell ,peptides ,anuran skin secretions ,rana-box ,Research Article ,Antimicrobial Cationic Peptides - Abstract
Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, Ranatuerin-2PLx (R2PLx), was identified from lyophilised skin secretions. The chemically synthetic replicates exhibited moderate and broadspectrum antimicrobial effect against various microorganisms including methicillin-resistant Staphylococcus aureus (MRSA, minimal inhibitory concentration = 256 µM). In addition, R2PLx was found to inhibit the proliferation of several tumour cells, especially showing more potent effect on prostate cancer cell, PC-3. The early cell apoptosis was observed in 6 h by Annexin V-FITC/propidium iodide staining, as well as the activation of Caspase-3 at 5 µM peptide concentration. R2PLx may therefore be promising for developing new therapeutic approach for cancer treatment. Moreover, the artificial deficiency of conserved rana-box loop or net positive charge in C-terminal domain notably reduced the biological activities of the truncated and substituted isoforms, respectively, suggesting for maintaining their biological potency of ranatuerin family requires both cysteine-bridged segment and cationincity within the loop domain in C-terminus.
- Published
- 2018