1. Highly sensitive electrochemical biosensor based on nonlinear hybridization chain reaction for DNA detection.
- Author
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Jia, Liping, Shi, Shanshan, Ma, Rongna, Jia, Wenli, and Wang, Huaisheng
- Subjects
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ELECTROCHEMICAL sensors , *ELECTRON-transfer catalysis , *DNA analysis , *CHEMICAL reactions , *CHEMICAL reduction - Abstract
In the present work we demonstrated an ultrasensitive detection platform for specific DNA based on nonlinear hybridization chain reaction (HCR) by triggering chain-branching growth of DNA dendrimers. HCR was initiated by target DNA ( t DNA) and finally formed dendritic structure by self-assembly. The electrochemical signal was drastically enhanced by capturing multiple catalytic peroxidase with high-ordered growth. Electrochemical signals were obtained by measuring the reduction current of oxidized 3, 3′, 5, 5′-tetramethylbenzidine sulfate (TMB), which was generated by HRP in the presence of H 2 O 2 . This method exhibited ultrahigh sensitivity to t DNA with detection limit of 0.4 fM. Furthermore, the biosensor was also capable of discriminating single-nucleotide difference among concomitant DNA sequences. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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