1. Signal amplification strategy for biomarkers: Structural origins of epitaxial-growth twinned nanocrystals and D–π–A type polymers.
- Author
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Liu, He, Gu, Yue, Dong, Tao, Yan, Liuqing, Yan, Xiaoyi, Zhang, Tingting, Lu, Nannan, Xu, Zhiqian, Xu, Haixin, Zhang, Zhiquan, and Bian, Ting
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AMPLIFICATION reactions , *BIOLOGICAL tags , *EPITAXY , *NANOCRYSTALS , *HYDROGEN peroxide reduction - Abstract
The combination of nanoparticles and biomarkers yields functional nanostructured biointerface, which is playing a notable role in biotechnology development. Due to the 5-fold twined structure in the Au-Pt star-shaped decahedra not only allowed it to act as efficient scaffold for immobilization of antibody, but it also exhibits superior electrocatalytic activity toward H 2 O 2 reduction, the nanocrystal as the efficient signal transduction label is first employed to construct an electrochemical immunosensor. Donor–π–Acceptor (D–π–A) linking fashion generates a dipolar push-pull system and assures superior intramolecular charge transfer. It is considered as a suitable π-conjugated backbone for conducting polymer on biointerface application. Under a D–π–A architecture which imidazole as the π-bridge and amino phenyl/phenyl groups as peripheral electron-donating/withdrawing functional groups, 4-(2,4,5-triphenyl-1H-imidazol-1-yl) aniline (TPIDA) is designed and synthesized for good biocompatibility and high conductivity. In this proposal, we attempt to integrate the above-mentioned two features from nanobiotechnology and organic bioelectronics. Then, a novel nonenzymatic sandwich-type immunosensor is performed by Au-Pt core-shell with surface-engineered twinning as a label and π-conjugated D–π–A polymers as the signal amplification platform. Human IgG (HIgG) as the model target protein can be detected with a wide linear range from 0.1 pg mL −1 to 100 ng mL −1 . The detection limit is down to 0.06 pg mL −1 (S/N = 3). Moreover, as a practical application, the prepared biosensor is used to monitor HIgG level in human serum with desirable results obtained. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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