1. Magnetic electrode-based electrochemical immunosensor using amorphous bimetallic sulfides of CoSnSx as signal amplifier for the NT[sbnd]pro BNP detection.
- Author
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Li, Yueyuan, Wang, Yaoguang, Zhang, Nuo, Fan, Dawei, Liu, Lei, Yan, Tao, Yang, Xinglong, Ding, Caifeng, Wei, Qin, and Ju, Huangxian
- Subjects
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CARBON electrodes , *MAGNETIC materials , *MAGNETISM , *SULFIDES , *BRAIN natriuretic factor , *COBALT chloride - Abstract
Abstract In this work, by using amorphous bimetallic sulfides of CoSnS x Pd as signal amplifier and magnetic material of Fe 3 O 4 @PPy Au as the substrate, we fabricated an electrochemical immunoassay for N terminal prohormone of brain natriuretic peptide (NT pro BNP). CoSnS x was used as the signal amplifier of the immunosensor for the first time, which was synthesized by fast stoichiometric co-precipitation method. The binary sulfide was identified as an amorphous structure and performed apparent electrochemical behavior. The excellent performance of signal marker was induced by the synergistic effect between SnS 2 and cobalt, as well as the loaded Pd nanoparticles (NPs). Both CoSnS x and Pd NPs presented excellent electrocatalytic activity toward H 2 O 2 oxidation. Moreover, the magnetic nanocomposite of Fe 3 O 4 @PPy Au was firmly immobilized on the magnetic glassy carbon electrode (MGCE) by the magnetic force. The magnetic substrate not only performed good electron conductivity, but also improved the stability of the fabricated electrochemical immunosensor. The developed immunosensor for the NT pro BNP detection exhibited a wide linear response (0.1 pg/mL to 50 ng/mL), and low detection limit of 31.5 fg/mL. In addition, the immunosensor held an excellent analysis capability and broad fascinating application for the detection of other biomarkers in medical diagnosis and treatment. Graphical abstract An electrochemical immunosensor based on amorphous bimetallic sulfides of CoSnS x Pd as signal amplifier and magnetic material of Fe 3 O 4 @PPy Au as the substrate for the NT pro BNP detection. fx1 Highlights • A novel electrochemical immunosensor was designed for detecting NT pro BNP. • CoSnS x was first used as the signal amplifier of the immunosensor and performed apparent electrochemical behavior. • The Fe 3 O 4 @PPy Au as matrix was firmly immobilized on the MGCE by the magnetic force. • It achieved an excellent result in human serum sample analysis. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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