1. Label-free low-cost disposable DNA hybridization detection systems using organic TFTs
- Author
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Zhang, Qintao, Jagannathan, Lakshmi, and Subramanian, Vivek
- Subjects
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NUCLEIC acid hybridization , *THIN film transistors , *DIAGNOSTIC reagents & test kits , *BIOSENSORS , *DNA , *MICROFLUIDICS , *PHOTOLITHOGRAPHY , *GENETIC disorder diagnosis - Abstract
Abstract: Organic thin film transistors (OTFT) are potentially very attractive for making portable and disposable DNA hybridization detection toolkits because of their ultra-low-cost manufacturing and potentially high sensitivity. To make a self-supported DNA sensor, we herein report an OTFT-based label-free DNA hybridization detection system integrating electrically read DNA hybridization sensors and microfluidic delivery channels. We previously reported DNA-doped OTFTs acting as electrically read DNA sensors. In this article, we first study physics of DNA sensitivity of OTFTs, clarifying the doping mechanism. The article also presents a method of verification of the immobilization and presence of DNA on the surface using TOF-SIMS analysis. As a further step, sources of variation of measured data and methods of minimization are discussed using a novel photolithography-based microfluidics fabrication method, directly enabling on-chip hybridization and self-supported DNA detection. By integrating the sensors with microfluidics, for the first time, we demonstrate necessary technologies required to realize disposable, rapid turn-around tools for field-deployable genetic diagnosis. [Copyright &y& Elsevier]
- Published
- 2010
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