1. Sum-frequency generation spectroscopy of DNA monolayers
- Author
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Paul Thiry, Gerard Tourillon, Alaa Addin Mani, Dan Lis, Laurent Dreesen, Yannick Sartenaer, André Peremans, Institut de recherches sur la catalyse et l'environnement de Lyon (IRCELYON), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
- Subjects
Tris ,Intercalation (chemistry) ,Biomedical Engineering ,Biophysics ,Analytical chemistry ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Monolayer ,Electrochemistry ,Molecule ,Spectroscopy ,"Sum-frequency generation " ,Chemistry ,Spectrum Analysis ,Sum-frequency generation ,Substrate (chemistry) ,"DNA" ,[CHIM.CATA]Chemical Sciences/Catalysis ,DNA ,General Medicine ,021001 nanoscience & nanotechnology ,[SDE.ES]Environmental Sciences/Environmental and Society ,0104 chemical sciences ,0210 nano-technology ,Platinum ,Biotechnology ,Sum frequency generation spectroscopy - Abstract
International audience; The anchoring of thiolated single-stranded DNA (HS-ssDNA) monolayers onto platinum substrates was investigated by sum-frequency generation spectroscopy. Different buffer solutions were used for the preparation of the adlayers. Vibrational fingerprints in the 2700–3100 cm−1 spectral range showed the intercalation of Tris/EDTA (TE) buffer molecules within the HS-ssDNA self-assembled monolayer. Buffer contribution to SFG can be quenched either by using SFG inactive molecules like KH2PO4/K2HPO4/NaCl (PBS) or by repeated rinsing of the DNA layer with pure water. Comparing the SFG spectra of HS-ssDNA and mercaptohexanol (MCH), which had been self-assembled onto the same substrate, enabled us to infer ordering of the anchor arms and strong disordering of the DNA strands of HS-ssDNA monolayers self-assembled on platinum.
- Published
- 2007
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