1. Rapid capture and labeling of cells on single domain antibodies-functionalized flow cell.
- Author
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Chen GY, Li Z, Duarte JN, Esteban A, Cheloha RW, Theile CS, Fink GR, and Ploegh HL
- Subjects
- Aminoacyltransferases analysis, Animals, Bacterial Proteins analysis, Candidiasis blood, Cysteine Endopeptidases analysis, Female, Flow Cytometry, Mice, Inbred C57BL, Mice, Transgenic, Nanostructures chemistry, Antibodies, Immobilized chemistry, Candida albicans isolation & purification, Cell Separation methods, Graphite chemistry, Leukocytes cytology, Leukocytes microbiology, Single-Domain Antibodies chemistry
- Abstract
Current techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of two functionalized graphene oxide (GO) surfaces that allow the capture of distinct leukocyte populations from small volumes blood using camelid single-domain antibodyfragments (VHHs) as capture agents. We used site-specifically labeled leukocytes to detect and identify cells exposed to fungal challenge. Combining the chemical and optical properties of GO with the versatility of the VHH scaffold in the context of a flow system provides a quick and efficient method for the capture and characterization of functional leukocytes., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2017
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