1. Electrochemical assay of the nitrate and nitrite reductase activities of Rhizobium japonicum.
- Author
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Salome JP, Amutha R, Jagannathan P, Josiah JJ, Berchmans S, and Yegnaraman V
- Subjects
- Enzyme Activation, Equipment Design, Equipment Failure Analysis, Rhizobium chemistry, Biosensing Techniques instrumentation, Electrochemistry instrumentation, Nitrate Reductase analysis, Nitrate Reductase chemistry, Nitrite Reductases analysis, Nitrite Reductases chemistry, Rhizobium enzymology
- Abstract
This work describes an electrochemical method for the determination of the nitrate and nitrite reductase activities of Rhizobium japonicum. The advantage of the method lies in the use of whole cells for the analysis and we earlier developed this protocol for the assay of NO. The results obtained are comparable to the spectrophotometric Griess assay. As the method is based on electrochemical reduction, the commonly interfering biological components like ascorbic acid, uric acid, dopamine, etc., will not interfere with the analysis. This method can be extended to the fabrication of biosensors for nitrate and nitrite using the same principle.
- Published
- 2009
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