1. Non-enzymatic electrochemical detection of glucose with a disposable paper-based sensor using a cobalt phthalocyanine-ionic liquid-graphene composite
- Author
-
Thai Long Hoang, Eda Mehmeti, Orawon Chailapakul, Sudkate Chaiyo, Kurt Kalcher, Weena Siangproh, and Hai Phong Nguyen
- Subjects
Blood Glucose ,Paper ,Materials science ,Indoles ,Scanning electron microscope ,Supporting electrolyte ,Inorganic chemistry ,Biomedical Engineering ,Biophysics ,Analytical chemistry ,Ionic Liquids ,Wine ,02 engineering and technology ,Biosensing Techniques ,01 natural sciences ,law.invention ,Electron transfer ,chemistry.chemical_compound ,law ,Limit of Detection ,Electrochemistry ,Organometallic Compounds ,Humans ,Electrodes ,Detection limit ,Graphene ,010401 analytical chemistry ,General Medicine ,Electrochemical Techniques ,Equipment Design ,Honey ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Glucose ,chemistry ,Transmission electron microscopy ,Ionic liquid ,Electrode ,Graphite ,0210 nano-technology ,Food Analysis ,Biotechnology - Abstract
We introduce for the first time a paper-based analytical device (PAD) for the non-enzymatic detection of glucose by modifying a screen-printed carbon electrode with cobalt phthalocyanine, graphene and an ionic liquid (CoPc/G/IL/SPCE). The modifying composite was characterized by UV-visible spectroscopy, energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The disposable devices show excellent conductivity and fast electron transfer kinetics. The results demonstrated that the modified electrode on PADs had excellent electrocatalytic activity towards the oxidation of glucose with NaOH as supporting electrolyte (0.1M). The oxidation potential of glucose was negatively shifted to 0.64V vs. the screen-printed carbon pseudo-reference electrode. The paper-based sensor comprised a wide linear concentration range for glucose, from 0.01 to 1.3mM and 1.3-5.0mM for low and high concentration of glucose assay, respectively, with a detection limit of 0.67µM (S/N = 3). Additionally, the PADs were applied to quantify glucose in honey, white wine and human serum. The disposable, efficient, sensitive and low-cost non-enzymatic PAD has great potential for the development of point-of-care testing (POCT) devices that can be applied in healthcare monitoring.
- Published
- 2017