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Your search keyword '"Raheleh, Rahbari"' showing total 2 results
Start Over Author "Raheleh, Rahbari" Journal biotechniques
2 results on '"Raheleh, Rahbari"'

1. A novel L1 retrotransposon marker for HeLa cell line identification

Author

Raheleh Rahbari, Tom Sheahan, Vasileios Modes, Pam Collier, Catriona Macfarlane, and Richard M. Badge

Subjects
cell culture, LINE-1, HeLa, contamination, genotyping, Biology (General), QH301-705.5
Abstract

The HeLa cell line is the oldest, most widely distributed, permanent human cell line. As a nearly ubiquitous inhabitant of laboratories using tissue culture techniques, its aggressive growth characteristics make it a problematic contaminant that can overgrow less robust cell lines. Consequently, HeLa contamination is common in both the research laboratory and cell line repository contexts, and its detection is hampered by the lack of a rapid, sensitive and robust assay. Here we report the development of a HeLa-specific DNA diagnostic test: a single duplex detection PCR assay targeting an L1 retrotransposon insertion. All HeLa clones from a geographically diverse panel were positive by this assay, and the particular L1 insertion we identified appears to be unique to the HeLa cell line. The assay can detect very low levels of HeLa contamination (

Published
2009
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2. A novel L1 retrotransposon marker for HeLa cell line identification

Author

Thomas W. Sheahan, Raheleh Rahbari, Richard M. Badge, Vasileios Modes, Catriona Macfarlane, and Pam Collier

Subjects
Cell, Retrotransposon marker, Retrotransposon, Polymerase Chain Reaction, Article, General Biochemistry, Genetics and Molecular Biology, law.invention, HeLa, Tissue culture, chemistry.chemical_compound, Gene Frequency, law, medicine, Humans, Cells, Cultured, Polymerase chain reaction, biology, biology.organism_classification, Molecular biology, Long Interspersed Nucleotide Elements, medicine.anatomical_structure, chemistry, Cell culture, Equipment Contamination, Biomarkers, DNA, HeLa Cells, Biotechnology
Abstract

The HeLa cell line is the oldest, most widely distributed, permanent human cell line. As a nearly ubiquitous inhabitant of laboratories using tissue culture techniques, its aggressive growth characteristics make it a problematic contaminant that can overgrow less robust cell lines. Consequently, HeLa contamination is common in both the research laboratory and cell line repository contexts, and its detection is hampered by the lack of a rapid, sensitive and robust assay. Here we report the development of a HeLa-specific DNA diagnostic test: a single duplex detection PCR assay targeting an L1 retrotransposon insertion. All HeLa clones from a geographically diverse panel were positive by this assay, and the particular L1 insertion we identified appears to be unique to the HeLa cell line. The assay can detect very low levels of HeLa contamination (

Published
2009

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