1. Modified ARCHITECT® serologic assays enable plasma-level performance from dried blood spot samples
- Author
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Vera Holzmayer, Mark Anderson, Nicaise Ndembi, Dora Mbanya, James Moy, and Gavin Cloherty
- Subjects
anti-HBc II ,dried blood spots ,HBeAg ,HBsAg NEXT ,HBV ,HIV Ag/Ab combo ,Biology (General) ,QH301-705.5 - Abstract
Dried blood spots (DBSs) provide an alternative sample input for serologic testing. We evaluated DBSs for the ARCHITECT® hepatitis B surface antigen (HBsAg) NEXT, hepatitis B e-antigen (HBeAg), anti-hepatitis B core antigen (anti-HBc II), HIV antigen/antibody (Ag/Ab) Combo and AdviseDx SARS-CoV-2 IgG II assays. Assay performance with DBSs was assessed with or without assay modification and compared with on-market assay with plasma samples. DBS stability was also determined. HBsAg NEXT and HIV Ag/Ab Combo assays using DBSs showed sensitivity and specificity comparable to that of on-market assays. Modified HBeAg, anti-HBc II and SARS-CoV-2 IgG II DBS assays achieved performance comparable to on-market assays. Use of DBSs as input for high-throughput serologic assays is expected to have significant implications for improving population surveillance and increasing access to diagnostic testing.
- Published
- 2022
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