1. T1N6_22 positively regulates Botrytis cinerea resistance but negatively regulates Pseudomonas syringae pv. tomato DC3000 resistance in Arabidopsis thaliana.
- Author
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Weng, Qiao-yun, Song, Jin-hui, Zhao, Ya-ting, Zheng, Xu, Huang, Cong-cong, Wang, Guan-yu, Zhang, Jing, Xing, Ji-hong, and Dong, Jin-gao
- Subjects
BOTRYTIS cinerea ,HALO-blight ,PSEUDOMONAS syringae ,ARABIDOPSIS thaliana genetics ,GENE expression - Abstract
T1N6_22, a short-chain dehydrogenase/reductase family protein, was identified as a positive regulator inArabidopsis thalianaresistance againstBotrytis cinereaandAlternaria brassicaein our preliminary study. In this study, we found that the expression levels of theT1N6_22gene were induced and up-regulated inA. thalianaecotype Columbia (Col-0) afterB. cinereaandPseudomonas syringaepv. tomato DC3000 inoculation. Compared with the Col-0 andt1n6_22/T1N6_22plants, the expression ofPAL,PR4,PPO,SODandCATgenes were down-regulated in thet1n6_22plants. In Col-0 plants treated with salicylic acid (SA) and the SA analogue benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH), the expression levels ofT1N6_22were significantly enhanced, whereas the expression levels ofT1N6_22were reduced by jasmonic acid treatment. Meanwhile, thet1n6_22mutant exhibited enhanced resistance, whereas the wild-type Col-0 and complemented plants (t1n6_22/T1N6_22) showed susceptibility toPstDC3000. After inoculation withB. cinereaandPstDC3000, the expression levels of defence-related genesPR1,PR3,PR5,NPR1andPDF1.2int1n6_22were significantly different from those in Col-0 andt1n6_22/T1N6_22plants. Taken together, theT1N6_22gene played a negative role inArabidopsisresistance toPstDC3000. TheT1N6_22gene may be involved in the regulation of salicylic acid and jasmonic-acid–signalling pathways to affect the resistance ofArabidopsistoB. cinereaandPstDC3000. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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