1. Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest
- Author
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Niamh Harraghy, David Calabrese, Pascal Liechti, Amar Rida, Valérie Le Fourn, Xuan Droz, Thierry Colombet, Etienne Lançon, Pierre-Alain Girod, and Nicolas Mermod
- Subjects
0106 biological sciences ,0301 basic medicine ,Chinese hamster ovary cell ,Microfluidics ,Cell ,Sorting ,A protein ,Bioengineering ,Cell sorting ,Biology ,01 natural sciences ,Applied Microbiology and Biotechnology ,Molecular biology ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,medicine.anatomical_structure ,Secretory protein ,010608 biotechnology ,medicine ,Secretion ,Biotechnology - Abstract
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it. When coupled to a cell colony imaging and picking device, this approach allowed the identification of CHO cell clones secreting a therapeutic protein at high levels that were not achievable without the cell sorting procedure. Biotechnol. Bioeng. 2017;114: 1791–1802. © 2017 Wiley Periodicals, Inc.
- Published
- 2017
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