Search

In order to investigate the expression differences of Lonicera caerulea fruit genes at different developmental stages（green ripening stage and ripening stage）and to explore the key metabolic pathways of L. caerulea during growth and development, the transcriptome data of L. caerulea at green ripening stage and ripening stage was compared by using high-throughput sequencing technology with‘ Zhongkelan No.1’ variety as the experimental object. The results showed that there were 40.32 Gb Clean Data, each sample had > 6.07 Gb Clean Data, the percentage of Q30 bases was 93.89% and above, and the GC content ranged from 46.32% to 49.38%. There were 3 247 differentially expressed genes detected in the fruits of the two developmental stages, including 1 642 up-regulated genes and 1 605 down-regulated genes. GO functional enrichment analysis revealed that the key regulatory genes during the development of L. caerulea were mainly involved in metabolism, cellular and cellular components and catalytic activity. KEGG enrichment analysis revealed that the differentially expressed genes were mainly enriched in plant hormone signaling, carbon metabolism and amino acid biosynthesis. These results provide a reference for further studies on the dynamic changes during fruit development of L. caerulea, and provide a theoretical basis for subsequent studies on the identification of functional genes and genetic diversity analysis of L. caerulea. [ABSTRACT FROM AUTHOR]