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1. Generation of reproductive transgenic pigs of a CRISPR-Cas9-based oncogene-inducible system by somatic cell nuclear transfer

Author

Kiyoung Eun, Seon‐Ung Hwang, Mirae Kim, Junchul David Yoon, Eunhye Kim, Hyerin Choi, Gahye Kim, Hee‐Young Jeon, Jun‐Kyum Kim, Jung Yun Kim, Nayoung Hong, Min‐Gi Park, Junseok Jang, Hyeon Ju Jeong, Sung Jin Kim, Bong‐Woo Ko, Sang Chul Lee, Hyunggee Kim, and Sang‐Hwan Hyun

Subjects
Male, Nuclear Transfer Techniques, Swine, General Medicine, Oncogenes, Fibroblasts, Applied Microbiology and Biotechnology, Animals, Genetically Modified, Gene Knockout Techniques, Molecular Medicine, Animals, Humans, Female, CRISPR-Cas Systems
Abstract

Alternative cancer models that are close to humans are required to create more valuable preclinical results during oncology studies. Here, a new onco-pig model via developing a CRISPR-Cas9-based Conditional Polycistronic gene expression Cassette (CRI-CPC) system to control the tumor inducing simian virus 40 large T antigen (SV40LT) and oncogenic HRAS

Published
2022

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