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Candida antarctica synthesised surface-active mannosylerythritol lipids at 46 g l−1 by adding 80 g soybean oil l−1 to the medium and maintaining the pO2 at 50% with an air flow rate 1 vvm. Two-stage culturing of C. antarctica avoided medium foaming but the yield of biosurfactants synthesis was 28 g l−1. The biosurfactants decreased the surface tension of water to 35 mN m−1.