1. [Untitled]
- Author
-
Masayoshi Tamura, Kazuhisa Ohtaguchi, Susumu Kajiwara, and Nahoko Takahashi
- Subjects
Reporter gene ,biology ,Streptomycetaceae ,fungi ,Bioengineering ,Bialaphos ,General Medicine ,biology.organism_classification ,Synechococcus ,Applied Microbiology and Biotechnology ,Microbiology ,chemistry.chemical_compound ,Transformation (genetics) ,Plasmid ,chemistry ,bacteria ,Streptomyces hygroscopicus ,Gene ,Biotechnology - Abstract
A novel cyanobacterial vector, pTT201, containing the bar gene encoding resistance to herbicides, bialaphos and phosphinothricin, was constructed. In Synechococcus sp. strain PCC7942-SPc, the bar gene was successfully expressed. Plasmid pTT201 increased a minimum inhibitory concentration for bialaphos 16-fold over Synechococcus sp. strain PCC7942-SPc without pTT201. The combination of the bialaphos as a selective agent and the transformation by bar gene serves as a photostable selection system for Synechococcus.
- Published
- 1999