1. [Untitled]
- Author
-
Virapong Prachayasittikul, Sarah Fredriksson, Wimol Petkanchanapong, and Leif Bülow
- Subjects
Protease ,Phage display ,Melioidosis ,Burkholderia pseudomallei ,Protease binding ,medicine.medical_treatment ,Virulence ,Bioengineering ,General Medicine ,Biology ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Applied Microbiology and Biotechnology ,Virology ,Microbiology ,medicine ,Protease inhibitor (pharmacology) ,Bacteria ,Biotechnology - Abstract
Burkholderia pseudomallei is a causative agent of melioidosis, a fatal community acquired septicemia in Southeast Asia and Northern Australia. A protease has been proposed to be one of the major pathogenic factors to play a significant role in melioidosis. We have used phage display technology to identify peptides binding to B. pseudomallei protease. By screening a constrained cyclic heptapeptide library, five independent clones with affinity to this protease were isolated and the amino acid sequences were determined. The cyclic heptapeptides from two of the phage clones (Cys-Phe-Phe-Met-Pro-His-Thr-Phe-Cys) were identical and showed the strongest phage-protease interaction as detected by ELISA. Four of the five selected phages at the amount of 1013 phages could inhibit B. pseudomallei protease activity by approximately 50%.
- Published
- 2000