1. Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51
- Author
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Aziz Akin Denizci, Hatice Korkmaz Güvenmez, and Secil Berna Kuzu
- Subjects
chemistry.chemical_classification ,Article Subject ,biology ,Strain (chemistry) ,Metal ions in aqueous solution ,fungi ,Bacillus ,biology.organism_classification ,Enzyme assay ,Microbiology ,Incubation period ,Enzyme ,chemistry ,Bacillus thuringiensis ,Chitinase ,biology.protein ,General Earth and Planetary Sciences ,Food science ,Research Article ,General Environmental Science - Abstract
This paper reports the isolation and identification of chitinase-producing Bacillus from chitin-containing wastes, production of a thermostable and alkaline chitinasese, and enzyme characterization. Bacillus thuringiensis subsp. kurstaki HBK-51 was isolated from soil and was identified. Chitinase was obtained from supernatant of B. thuringiensis HBK-51 strain and showed its optimum activity at 110°C and at pH 9.0. Following 3 hours of incubation period, the enzyme showed a high level of activity at 110°C (96% remaining activity) and between pH 9.0 and 12.0 (98% remaining activity). Considering these characteristics, the enzyme was described as hyperthermophile-thermostable and highly alkaline. Two bands of the enzyme weighing 50 and 125 kDa were obtained following 12% SDS-PAGE analyses. Among the metal ions and chemicals used, Ni2+ (32%), K+ (44%), and Cu2+ (56%) increased the enzyme activity while EDTA (7%), SDS (7%), Hg2+ (11%), and ethyl-acetimidate (20%) decreased the activity of the enzyme. Bacillus thuringiensis subsp. kurstaki HBK-51 is an important strain which can be used in several biotechnological applications as a chitinase producer.
- Published
- 2012
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