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Your search keyword '"Mukhamedova, N"' showing total 4 results
Start Over Author "Mukhamedova, N" Journal biulleten eksperimentalnoi biologii i meditsiny
4 results on '"Mukhamedova, N"'

2. [The conjugation of preliminarily immunosorbent-immobilized 5B4D6 monoclonal antibodies with a chelating polymer].

Author

Mukhamedova NM and Slinkin MA

Subjects
Animals, Antibody Specificity, Binding Sites, Antibody, Drug Interactions, Glial Fibrillary Acidic Protein immunology, Humans, Immunoglobulin lambda-Chains immunology, Immunosorbents, Rabbits, Antibodies, Monoclonal chemistry, Chelating Agents chemistry, Pentetic Acid chemistry, Polylysine chemistry, Polymers chemistry
Abstract

A study has been performed to investigate the change in antigen-binding capacity of antibodies as a result of their interaction with chelating agents, and polymers under various conditions. It has been demonstrated that antibody immobilization on the sorbent preceded by the antibody conjugation with chelating polymer allows better maintenance of specific activity of 5B4D6 monoclonal antibodies. Such modification yields a 10-fold increase in the antibody-antigen binding as compared with a standard conjugation technique in a mixture.

Published
1992

3. [The selective accumulation of monoclonal antibodies in the lungs after cyclophosphane administration to rats].

Author

Torchilin VP, Mukhamedova NM, Il'ina AI, Iakubov LZ, and Shchitkov KG

Subjects
Animals, Antibodies, Monoclonal metabolism, Dose-Response Relationship, Drug, Iodine Radioisotopes, Lung metabolism, Male, Rats, Rats, Wistar, Tissue Distribution drug effects, Antibodies, Monoclonal drug effects, Cyclophosphamide administration & dosage, Lung drug effects
Abstract

The study has demonstrated the possibility to use cyclophosphamide as a model of "impairment" agent for lung tissue cells to provide access of monoclonal antibodies to nuclear antigen structures. Biodistribution of monoclonal antibodies to various intracellular antigens was studied on Wistar rats pretreated with various doses of cyclophosphamide. Accumulation of 2C5 antibodies to cell nuclei was found to be dependent on the dose of cyclophosphamide administered.

Published
1992

4. [The chemical modification of immunoglobulins for accelerating their elimination from the blood flow during radioimmunodiagnosis].

Author

Mukhamedova NM, Obukhov SK, Slinkin MA, Klibanov AL, and Torchilin VP

Subjects
Antibodies, Neoplasm analysis, Antibody Specificity immunology, Humans, Immunoglobulins analysis, Neoplasms diagnosis, Avidin, Biotin, Immunoglobulins isolation & purification, Radioimmunoassay methods
Abstract

Immunoglobulins were modified by diethylenetriaminepentaacetic acid anhydride and sulfosuccinimidy 16-(biotinamido) hexanoate and were conjugated with modified polylysine. Biodistribution of the samples was observed before and after avidin injection. Samples containing no polylysine accumulate in reticuloendothelial system, protein conjugate with polylysine concentrate in kidneys. The results demonstrate that drug distribution depends on the type of modification.

Published
1989

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