1. [Immunogenicity of H-antigen polymers obtained by different methods].
- Author
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Liashenko VL, Aleksander SK, Vaneeva LI, and Semenova GB
- Subjects
- Animals, Dose-Response Relationship, Immunologic, Flagellin immunology, Flagellin isolation & purification, Mice, Polymers isolation & purification, Salmonella typhi immunology, Antibody Formation, Antigens, Bacterial isolation & purification, Flagella immunology, Polymers immunology
- Abstract
The authors studied the properties of two polymeres prepared from flagellin by various methods: precipitation with ammonium sulfate with the subsequent centrifugation or treatment with glutaric aldehyde in solution and subsequent gel-chromatography. Molecules of the former (P-I) and of the latter (P-II) were similar by shape, but the mol wt of P-I was greater. The preparations contained common H-antigen and were similar by serological activity. P-I had a very high "priming" activity for mice. P-II was highly immunogenic only within the narrow range of doses, was less immunogenic in comparison with P-II, and, in difference from P-I, induced formation of some amount of 7S-antibodies.
- Published
- 1978