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Your search keyword '"Repetitive Sequences, Nucleic Acid drug effects"' showing total 2 results
Start Over Descriptor "Repetitive Sequences, Nucleic Acid drug effects" Journal biulleten eksperimentalnoi biologii i meditsiny
2 results on '"Repetitive Sequences, Nucleic Acid drug effects"'

1. [Increased content of nucleotide sequences in transcriptionally active DNA and poly(A)+-mRNA of the rat liver and a rise in its translation activity as affected by inducers].

Author

Dashkevich VS, Vishnivetskiĭ SN, Skobel'tsina LM, Luk'ianchikova NL, and Kaledin VI

Subjects
Adrenal Glands physiology, Animals, Base Sequence drug effects, DNA genetics, Hydrocortisone pharmacology, Liver metabolism, Male, Methyldimethylaminoazobenzene pharmacology, Nucleic Acid Hybridization drug effects, Rats, Rats, Inbred Strains, Repetitive Sequences, Nucleic Acid drug effects, DNA drug effects, Liver drug effects, Poly A genetics, Protein Biosynthesis drug effects, RNA, Messenger genetics, Transcription, Genetic drug effects
Abstract

Cortisol and 3'-methyl-4-dimethyl-amino-azobenzene induce an increase in the content of repeated sequences (RS) in transcriptionally active (TA) DNA, while the content of respective RS in potentially active DNA fractions enriched with regulatory regions of the genome decreases. RS content in induced poly A+-mRNA also rises, as determined by the nature of hybridization of respective c DNA with total DNA. The translation of induced poly A+-mRNA rises essentially, with the qualitative distinctions in in vitro synthesized protein product spectrum being absent. Inducible RS with unstable chromatine conformation are thought to provide a universal system of rapid response of the genetic apparatus to extreme situations, serving as transcription intensifiers in TA DNA and as translation intensifiers in induced poly A+-mRNA.

Published
1986

2. [Detection of DNA sequences sensitive to glucocorticoids in the thymus of AKR mice and their inactivation in thymoma].

Author

Novikova VM, Dashkevich VS, Vishnivetskiĭ SN, Kaledin VI, and Mertvetsov NP

Subjects
Animals, DNA genetics, DNA, Neoplasm genetics, Hydrocortisone pharmacology, Mice, DNA analysis, Glucocorticoids pharmacology, Mice, Inbred AKR genetics, Repetitive Sequences, Nucleic Acid drug effects, Thymoma genetics, Thymus Gland analysis
Abstract

A high increase in transcriptionally active DNA fraction (TA DNA) has been detected in the thymus of young AKR mice and in thymoma. Hybridization of TA DNA with [32P] cDNA synthesized on poly A+-mRNA of cortisol-induced animals has shown that TA DNA of young AKR mice contains 40 times as much cortisol-activated repeated sequences (RS) as that of thymoma. The decrease of cortisol-activated (RS) in AKR thymoma TA DNA was found to be the result of their transition into transcriptionally inactive (TI) DNA. It is concluded that chromatin conformation changes take place in cortisol-activated RS DNA region of AKR mouse thymus before transformation. Unstable conformation of RS DNA in the thymoma can possibly promote their transition into TI DNA.

Published
1986

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