1. Sodium butyrate and a T lymphocyte cell line-derived differentiation factor induce basophilic differentiation of the human promyelocytic leukemia cell line HL-60.
- Author
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Hutt-Taylor SR, Harnish D, Richardson M, Ishizaka T, and Denburg JA
- Subjects
- Biological Products metabolism, Butyric Acid, Cell Differentiation drug effects, Cell Line, Cytokines, Drug Synergism, Histamine Release, Humans, Male, Receptors, Fc analysis, Receptors, IgE, Tumor Cells, Cultured pathology, Basophils analysis, Biological Products pharmacology, Butyrates pharmacology, Leukemia, Myeloid, Acute pathology, T-Lymphocytes metabolism, Tumor Cells, Cultured drug effects
- Abstract
Sodium butyrate induces basophilic differentiation of HL-60 promyelocytic leukemia cells that have been previously passaged in alkaline medium. A factor present in Mo conditioned medium (Mo-CM) acts synergistically with sodium butyrate to promote basophilic maturation in a dose-dependent fashion. The induced HL-60 cells exhibit nuclei at various stages of maturity and cytoplasmic granules staining azurophilic with May-Grünwald-Giemsa and metachromatically with toluidine blue. The histamine content of induced HL-60 cells is 50 ng/10(6) cells with sodium butyrate alone or 190 ng/10(6) cells with butyrate in combination with Mo-CM. Induced cells release histamine in response to anti-IgE and have receptors for the Fc portion of human IgE. The basophilic cell-differentiating activity present in Mo-CM appears to be distinct from several other cytokines including recombinant human interleukin-1 alpha, interleukin-2, interferon-gamma, interferon-alpha, murine interleukin-3, erythroid-potentiating activity, and purified human granulocyte/macrophage colony-stimulating factor. This is the first demonstration of a cell line that is capable of differentiation along the basophil lineage and could provide a useful model for examining biochemical and molecular events associated with basophil differentiation.
- Published
- 1988