1. Isolation of an early T-cell precursor (CFU-TL) from human bone marrow
- Author
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G. Mouterde, JM Bertho, A H Dalloul, Patrice Debré, and M D Mossalayi
- Subjects
Antigens, Differentiation, T-Lymphocyte ,Time Factors ,medicine.drug_class ,T-Lymphocytes ,T cell ,Population ,Immunology ,CD2 Antigens ,Bone Marrow Cells ,Antigens, CD7 ,Cell Separation ,Biology ,Monoclonal antibody ,Cell morphology ,Biochemistry ,Flow cytometry ,Colony-Forming Units Assay ,Antigens, CD ,medicine ,Humans ,Receptors, Immunologic ,education ,education.field_of_study ,medicine.diagnostic_test ,Cell Differentiation ,Cell Biology ,Hematology ,Flow Cytometry ,Hematopoietic Stem Cells ,Molecular biology ,In vitro ,medicine.anatomical_structure ,Cell culture ,Bone marrow ,Cell Division - Abstract
CD2-CD3-CD4-CD8- human bone marrow (BM) cells were previously shown to generate T-cell clones in vitro. This capacity was abolished after treatment of this population with anti-CD7 monoclonal antibody and complement. In this study, using rosetting with sheep erythrocytes, complement-dependent cytotoxicity, and specific immunoadherence method, we isolated a minor BM subset that contained more than 80% CD7+CD2-CD3- CD4-CD8- cells with small lymphoid cell morphology. They comprised most early T-cell precursors (CFU-TL) as they displayed high capacity to generate T-cell clones when cultured in limiting dilutions. CFU-TL nature of these cells was also confirmed by the sequential expression of mature T-cell specific markers on their surface after in vitro induction. This BM subset also contained 2% to 3% CFU-GM precursors. Together, these results pointed to the existence of BM CD7+CD2- precursors with high differentiation potential and showed the commitment of most of them to T-cell lineage.
- Published
- 1990
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