Search

Your search keyword '"Ito, Satoshi"' showing total 8 results
Start Over Author "Ito, Satoshi" Journal blood
8 results on '"Ito, Satoshi"'

8. Interleukin-10 Inhibits Expression of Both Interferon ?– and Interferon ?– Induced Genes by Suppressing Tyrosine Phosphorylation of STAT1

Author

Ito, Satoshi, Ansari, Parswa, Sakatsume, Minoru, Dickensheets, Harold, Vazquez, Nancy, Donnelly, Raymond P., Larner, Andrew C., and Finbloom, David S.

Abstract

Interleukin-10 (IL-10) helps maintain polarized T-helper cells in a T-helper lymphocyte 2 (Th2) phenotype. Part of this process involves the prevention of the development of Th1 cells, which are a primary source of interferon ? (IFN?), a potent activator of monocytes and an inhibitor of Th2 proliferation. Because monocytes and macrophages are important mediators of Th1-type responses, such as delayed-type hypersensitivity, we sought to determine if IL-10 could directly mediate inhibition of IFN?- and IFN?-induced gene expression in these cells. Highly purified monocytes were incubated with IL-10 for 60 to 90 minutes before the addition of IFN? or IFN?. IL-10 preincubation resulted in the inhibition of gene expression for several IFN-induced genes, such as IP-10, ISG54, and intercellular adhesion molecule-1. The reduction in gene expression resulted from the ability of IL-10 to suppress IFN-induced assembly of signal transducer and activator of transcription (STAT) factors to specific promoter motifs on IFN?- and IFN?-inducible genes. This was accomplished by preventing the IFN-induced tyrosine phosphorylation of STAT1, a component of both IFN?- and IFN?-induced DNA binding complexes. Therefore, IL-10 can directly inhibit STAT-dependent early response gene expression induced by both IFN? and IFN? in monocytes by suppressing the tyrosine phosphorylation of STAT1. This may occur through the ability of IL-10 to induce expression of the gene, suppressor of cytokine signaling 3 (SOCS3).

Published
1999
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results