1. Glucose-6-phosphate dehydrogenase (G6PD) mutations and UDP-glucuronosyltransferase promoter polymorphism among G6PD deficient Kuwaitis.
- Author
-
Samilchuk E, Al-Suliman I, Usanga E, and Al Awadi S
- Subjects
- Gene Frequency, Glucosephosphate Dehydrogenase blood, Glucosephosphate Dehydrogenase Deficiency blood, Glucuronosyltransferase blood, Humans, Kuwait, Male, Glucosephosphate Dehydrogenase genetics, Glucosephosphate Dehydrogenase Deficiency genetics, Glucuronosyltransferase genetics, Point Mutation, Polymorphism, Genetic, Promoter Regions, Genetic
- Abstract
Screening of 1,080 Kuwaiti male blood donors for glucose-6-phosphate dehydrogenase (G6PD) deficiency revealed this condition in 70 (6.5%) individuals. Mutation analysis of all 70 G6PD deficient samples performed by PCR/RFLP and direct sequencing identified the 563C-->T (Mediterranean) in 72.9%, 202G-->A (A(-)) in 14.3%, 1003G-->A (Chatham) in 7.1%, and 143T-->C (Aures) in 1.4%. In 3 cases (4.3%) mutations remain unknown. Genotyping of all G6PD deficient samples for UDP-glucuronosyltransferase 1 (UDPGT1) gene promoter polymorphism revealed (ta)6/(ta)6 in 38.6%, (ta)7/(ta)7 in 15.7%, (ta)6/(ta)7 in 44.3%, and (ta)7/(ta)8 allele in 1.4% of cases. Thus, 4% of males in the Kuwaiti population have G6PD deficiency coexisting with low activity of the UDPGT1 promoter.
- Published
- 2003
- Full Text
- View/download PDF