1. Low-dose metronomic doxorubicin inhibits mobilization and differentiation of endothelial progenitor cells through REDD1-mediated VEGFR-2 downregulation
- Author
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Joohwan Kim, Young-Guen Kwon, Ji Yoon Kim, Minsik Park, Young-Myeong Kim, and Jeong-Hyung Lee
- Subjects
Male ,Vascular Endothelial Growth Factor A ,Angiogenesis ,medicine.medical_treatment ,Transplantation, Heterologous ,Melanoma, Experimental ,Down-Regulation ,Bone Marrow Cells ,mTORC1 ,Mechanistic Target of Rapamycin Complex 1 ,Nitric Oxide ,Biochemistry ,Article ,Mice ,Downregulation and upregulation ,medicine ,Animals ,Epidermal growth factor receptor ,Progenitor cell ,Molecular Biology ,Cells, Cultured ,Endothelial Progenitor Cells ,Mice, Knockout ,Vascular Endothelial Growth Factor Receptor-1 ,Neovascularization, Pathologic ,biology ,Chemistry ,Metronomic chemotherapy ,Growth factor ,REDD1 ,Cell Differentiation ,General Medicine ,Vascular Endothelial Growth Factor Receptor-2 ,Metronomic Chemotherapy ,VEGFR-2 ,Doxorubicin ,Tumor progression ,EPCs ,embryonic structures ,cardiovascular system ,Cancer research ,biology.protein ,Transcription Factors - Abstract
Low-dose metronomic chemotherapy has been introduced as a less toxic and effective strategy to inhibit tumor angiogenesis, but its anti-angiogenic mechanism on endothelial progenitor cells (EPCs) has not been fully elucidated. Here, we investigated the functional role of regulated in development and DNA damage response 1 (REDD1), an endogenous inhibitor of mTORC1, in low-dose doxorubicin (DOX)-mediated dysregulation of EPC functions. DOX treatment induced REDD1 expression in bone marrow mononuclear cells (BMMNCs) and subsequently reduced mTORC1-dependent translation of endothelial growth factor (VEGF) receptor (Vegfr)-2 mRNA, but not that of the mRNA transcripts for Vegfr-1, epidermal growth factor receptor, and insulin-like growth factor-1 receptor. This selective event was a risk factor for the inhibition of BMMNC differentiation into EPCs and their angiogenic responses to VEGF-A, but was not observed in Redd1-deficient BMMNCs. Low-dose metronomic DOX treatment reduced the mobilization of circulating EPCs in B16 melanoma-bearing wild-type but not Redd1-deficient mice. However, REDD1 overexpression inhibited the differentiation and mobilization of EPCs in both wild-type and Redd1-deficient mice. These data suggest that REDD1 is crucial for metronomic DOX-mediated EPC dysfunction through the translational repression of Vegfr-2 transcript, providing REDD1 as a potential therapeutic target for the inhibition of tumor angiogenesis and tumor progression. [BMB Reports 2021; 54(9): 470-475].
- Published
- 2021
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