1. Upregulation of heme oxygenase-1 in colorectal cancer patients with increased circulation carbon monoxide levels, potentially affects chemotherapeutic sensitivity
- Author
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Hongzhuan Yin, Qi Su, Long Liao, Hiroshi Maeda, and Jun Fang
- Subjects
Male ,Cancer Research ,Pathology ,Colorectal cancer ,Gene Expression ,chemistry.chemical_compound ,Mice ,Surgical oncology ,Neoplasm Metastasis ,Carbon monoxide ,Aged, 80 and over ,Middle Aged ,Immunohistochemistry ,Tumor Burden ,Protein Transport ,Oncology ,Carboxyhemoglobin ,Heme oxygenase-1 ,Female ,Colorectal Neoplasms ,HT29 Cells ,medicine.drug ,Research Article ,Adult ,medicine.medical_specialty ,Pirarubicin ,Antineoplastic Agents ,Cell Line, Tumor ,Genetics ,medicine ,Animals ,Humans ,Viability assay ,Chemosensitivity ,Aged ,Neoplasm Staging ,business.industry ,Zinc protoporphyrin ,Cancer ,medicine.disease ,Xenograft Model Antitumor Assays ,digestive system diseases ,Heme oxygenase ,Disease Models, Animal ,chemistry ,Drug Resistance, Neoplasm ,Cancer research ,business - Abstract
Background Heme oxygenase-1 (HO-1) and its major product carbon monoxide (CO) are known to be involved in the development and progression of many tumors. The present study was to elucidate the expression and function of HO-1 in colorectal cancer (CRC), specially focusing on the circulation CO levels in CRC patients and the possible roles of HO-1 in chemoresistance of colon cancer cells. Methods One hundred and eighteen patients received resection for colorectal cancer and polyps at China Medical University Sheng Jing Hospital, were collected in this study. HO-1 expression in CRC tissues was analyzed by immnuohistochemical staining; circulation CO levels as carboxyhemoglobin (COHb) in CRC patients were analyzed by an ABL800 FLEX blood gas analyzer. HO-1 expression in murine colon cells C26 and human colon cancer cells HT29 and DLD1 under HO-1 inducer hemin and anticancer drug pirarubicin (THP) treatment was examined by RT-PCR, and the cell viability after each treatment was investigated by MTT assay. Data were analyzed by student’s t-test or one-way ANOVA followed by Bonferroni t-test or Fisher's exact test. Results HO-1 expression in tumor tissues of CRC (61.0%) was significantly higher than in normal colorectal tissues and polyps tissues (29.7%, P
- Published
- 2014