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Start Over Author "Yong Wang" Topic cell proliferation Journal bmc cell biology
2 results on '"Yong Wang"'

1. GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules.

Author

Yong Wang, Wei Song, Shuchun Li, Xin Guan, Shiying Miao, Shudong Zong, Koide, S. S., and Linfang Wang

Subjects
*APOPTOSIS, *SPERMATOGENESIS, *PROTEINS, *CELLS, *CELL proliferation
Abstract

Background: Apoptosis is important for regulating spermatogenesis. The protein mRHBDD1 (mouse homolog of human RHBDD1)/rRHBDD1 (rat homolog of human RHBDD1) is highly expressed in the testis and is involved in apoptosis of spermatogonia. GC-1, a spermatogonia cell line, has the capacity to differentiate into spermatids within the seminiferous tubules. We constructed mRHBDD1 knockdown GC-1 cells and evaluated their capacity to differentiate into spermatids in mouse seminiferous tubules. Results: Stable mRHBDD1 knockdown GC-1 cells were sensitive to apoptotic stimuli, PS341 and UV irradiation. In vitro, they survived and proliferated normally. However, they lost the ability to survive and differentiate in mouse seminiferous tubules. Conclusion: Our findings suggest that mRHBDD1 may be associated with mammalian spermatogenesis. [ABSTRACT FROM AUTHOR]

Published
2009
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2. GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules

Author

Yong Wang, Shiying Miao, Shuchun Li, Linfang Wang, Shohei Koide, Shudong Zong, Wei Song, and Xin Guan

Subjects
Male, endocrine system, Ultraviolet Rays, Recombinant Fusion Proteins, Blotting, Western, Green Fluorescent Proteins, Apoptosis, Biology, Transfection, Cell Line, Bortezomib, Mice, Testis, Animals, Regeneration, lcsh:QH573-671, RNA, Small Interfering, Spermatogenesis, Cell Proliferation, Gene knockdown, Mice, Inbred BALB C, lcsh:Cytology, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Membrane Proteins, Cell Biology, Seminiferous Tubules, Flow Cytometry, Boronic Acids, In vitro, Spermatogonia, Cell biology, ErbB Receptors, Cell culture, Gene Knockdown Techniques, Pyrazines, Research Article
Abstract

Background Apoptosis is important for regulating spermatogenesis. The protein mRHBDD1 (mouse homolog of human RHBDD1)/rRHBDD1 (rat homolog of human RHBDD1) is highly expressed in the testis and is involved in apoptosis of spermatogonia. GC-1, a spermatogonia cell line, has the capacity to differentiate into spermatids within the seminiferous tubules. We constructed mRHBDD1 knockdown GC-1 cells and evaluated their capacity to differentiate into spermatids in mouse seminiferous tubules. Results Stable mRHBDD1 knockdown GC-1 cells were sensitive to apoptotic stimuli, PS341 and UV irradiation. In vitro, they survived and proliferated normally. However, they lost the ability to survive and differentiate in mouse seminiferous tubules. Conclusion Our findings suggest that mRHBDD1 may be associated with mammalian spermatogenesis.

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